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KMID : 1059519920360020218
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Journal of the Korean Chemical Society
1992 Volume.36 No. 2 p.218 ~ p.222
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Plant Tissue Sensor for Determination of Cytidine
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Ihn Gwon-Shik
Chung Chin-Kap
Kim Bong-Weon
Jeon Young-Guk
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Abstract
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Rose tissue containing cytidine deaminase converts cytidine to uridine and ammonia gas. Rose tissue sensor was constructed by immobilizing 50mg of a rose petal tissue on an NH3 gas sensor and the optimum condition of the sensor for the determination of cytidine was investigated. The tissue sensor showed a linear range of 7.0 × 10-4∼1.0 × 10-2M cytidine with a slope of 53 mV/decade in 0.2 M phosphate buffer, pH 8.4 at 37℃. The detection limits were 3.0 × 10-4 M and relative standard deviation was 3.4%. This sensor showed an excellent selectivity among various nucleosides and amino acids.
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